Biochem 651 - Introduction to modern biochemical laboratory techniques.

    

Fall 2009-2010



 
  Description  
 
  Calendar  
 
  Course Materials  
 
  Contact  
 
 

Course Description:

Section 001 (Class Number: 70532 )
Instructor(s): Fox,Brian G.; Gillian,Anne Lynn; Senes,Alessandro
TA(s):
Credits: 2
Day: MWF   Time: 12:05 PM - 12:55 PM
Location: 184 Russell Labs   Website: http://www.biochem.wisc.edu/courses/biochem651/

Description:

Students currently enrolled in Biochem 651:

Please find the complete course syllabus and course materials on Learn@UW.


BIOCHEMISTRY 651, Biochemical Methods, is an integrated lecture and lab course covering basic theories and techniques used in the experimental life sciences. The course is directed at upper level undergraduate majors in Biochemistry. Participants will learn how to apply a broad range of biochemical, genetic, and physical techniques to modern biochemical research.

Lectures are scheduled such that theoretical and practical aspects are introduced before the corresponding lab experiments. The laboratory experiments are designed to provide "hands-on" experience with instruments and techniques that are frequently used in both biochemical and clinical research. These experiments focus on instrumental techniques including spectrophotometry, gel electrophoresis, enzyme assays, and protein purification. Students will begin the course by PCR amplifying and cloning the bacterial lac z gene. As the semester progresses the lac z clone will be used to over express β-galactosidase which will then be purified and assayed for functionality. The lab reports will be graded on the basis of data collection and analysis, and on their clarity, accuracy, and detail.

Two exams will cover the lecture materials and related lab experiments, and will contain both multiple choice and short essay questions.


Lectures and Laboratory Experiments:
  • Amplification of a DNA segment by PCR
  • Molecular cloning of the lac z gene
  • Analytical electrophoresis of proteins and nucleic acid
  • UV-visible spectrometry and spectroscopy in biochemical research
  • Assays to monitor enzyme activity
  • Affinity purification of over expressed protein
    


Discussions/Labs/Other:

LAB - 301 (Class Number: 70552 )
Instructor(s): Fox,Brian G.; Gillian,Anne Lynn; Senes,Alessandro
TA(s):
Credits: 2
Day: M
Time: 01:20 PM - 05:00 PM
Location: 2145 MBS
LAB - 302 (Class Number: 70553 )
Instructor(s): Fox,Brian G.; Gillian,Anne Lynn; Senes,Alessandro
TA(s):
Credits: 2
Day: T
Time: 09:55 AM - 01:35 PM
Location: Rm 2145 MSB
LAB - 303 (Class Number: 71646 )
Instructor(s): Fox,Brian G.; Gillian,Anne Lynn; Senes,Alessandro
TA(s):
Credits: 2
Day: W
Time: 01:20 PM - 05:00 PM
Location: Rm 2145 MSB

 

 

 

Copyright 2009 – This page last modified 11/23/2007

Website Feedback, questions or other issues