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Biochem 660 - BIOCHEMICAL TECHNIQUES
Fall 2008-2009
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Course Description:
Section 001
(Class Number: 10559
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| Instructor(s): |
Wickens,Marvin P. |
| TA(s): |
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| Credits: |
2
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| Day: |
MW
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Time: |
09:55 AM - 10:45 AM
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| Location: |
Rm 175 BCHM ADDN
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Website: |
http://www.biochem.wisc.edu/courses/biochem660/
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Description:
Aims
The course will focus on modern and emerging approaches to biochemical problems. We will discuss how the methods work, with an emphasis on their actual practice at the bench, how you do them, what are the pitfalls, and what are the strengths. We will emphasize what is available on campus.
Scheduling
We will meet on Mondays and Wednesdays at 9:55 am in Room 179 throughout the semester. In addition, a faculty discussion will take place at 5pm on Tuesday, September 23. During the Molecular Graphics and Bioinformatics Module, beginning on October 23, we will have sessions on Thursdays, 1:20 - 3:30 pm.
Lecturers
Lectures generally will be given by dissertators who volunteer and have expertise in the relevant methods. Dr. Wickens will oversee the course, and work with the lecturers. H. Adam Steinberg of the Media Center will oversee the Graphics and Presentation portion, and Drs. Ann Palmenberg and Jean-Yves Sgro will oversee the Molecular Graphics and Bioinformatics (MGB) portion of the course.
We will also have field trips to facilities in the department and on campus. These will take place at the usual class time. Specific meeting arrangements will be discussed in the preceding session.
To view photos and email addresses for the graduate teaching assistants, click here.
Reading materials
Reading will be available from course calendar on the Thursday before the lecture. Some will be required, and some will be optional. Be sure to look for any relevant comments on the web site.
Grade
There will be four exams (see calendar). Together, these will account for 60% of the grade. An oral presentation in the MGB module will be graded (30%). Class participation (10%) will also count toward the final grade.
Web site
Check the calendar to:
- Download required reading (look for any relevant comments about the reading)
- Download the lecture's visual aids (i.e., the images for a Power Point presentation) and bring them to class.
View
Calendar as Syllabus
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NOTE: Only
one(1) color is used to display on the calendar. The event with the
highest importance will be the color of the day. Click on the day or select the
type of event from they key to display ALL items of a particular type.
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Tuesday, October 07, 2008 (10/7/2008) |
No calendar events for today
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Course Materials
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Handout
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Conventional Methods of Protein Purification; PDF slides, 4/pg
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Exam 1
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FRET Lecture; PDF, 4 slides/pg
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Lecture slides: PDF, 4/pg
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Lecture slides; 4/pg
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Methods in Membrane Biochemistry; PDF slides, 4/pg
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Microscopy Lecture; ppt
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Protein Characterization via Mass Spec; PDF slides; 4 per page
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Protein purification
lecture notes
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Standard Chemical Shifts; PDF
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Tables from Conventional....pdf
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Testing Gene Function; pdf slides, 4/pg
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Reading
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Achieving In-Depth Proteomics Profiling by Mass Spectrometry
Ahn et al.
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Betzig, 2006
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Conventional Protein Purification and Characterization
Current Protocols in Protein Science
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Detergents as Tools in Membrane Biochemistry
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Doyle et al
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Gavin, 2002
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Hell, 2006
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Mullen, 2006
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Nagy et al
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Piston, et al
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Puig, 2002
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Reading 1: "Handbook"
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Reading 1; NMR history and applications (not required)
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Reading 2: NMR ligand binding (not required)
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Reading 2: Arnau, et al.
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Sorting out small RNAs, Kim, 2008
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Suggested reading
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Willig, 2006
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No homework assignments are currently due.
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