Regulation of plant development; mechanisms of floral induction
The major developmental change in the plant life cycle is the initiation of flowering. Many plant species have evolved the ability to regulate flowering in response to environmental variables such as changes in day-length or temperature. An example of a temperature response is that certain plants require relatively long periods of cold exposure during winter to initiate flowering the following spring. This acquisition of competence to flower after prolonged exposure to cold is known as vernalization. Before competence is achieved, plants must measure exposure to a sufficient number of days of cold to represent a complete winter; this ensures flowering only occurs when spring has arrived, rather than during a temporary warming in the middle of winter.
We are using a combination of genetics and biochemistry to better understand, at a molecular level, the process of vernalization. For example, we identified a gene (FLC) in the crucifer Arabidopsis thaliana that prevents flowering unless they have experienced the cold of winter. Exposure to cold promotes flowering via a stable epigenetic switch of FLC to a repressed state. This epigenetic state of FLC is reset to an active state in the next generation. We are also studying vernalization in the grass Brachypodium distachyon. Grasses have a vernalization system that evolved independently of that in Arabidopsis thaliana. In fact, the vernalization systems in different groups of plants all result from convergent evolution. This is not surprising because when flowering plants were diverging, the earth was much warmer and the landmasses were in different locations than at present. Only after many ecosystems became colder, would a vernalization response (and other cold responses such as cold-mediated release of bud dormancy) be adaptive.
We are also studying biomass traits in Brachypodium distachyon in an effort supported by the Great Lakes Bioenergy Research Center (www.glbrc.org/), and have several projects in the area of science education.
This picture shows a cabbage plant that was grown in the greenhouse for 5 years. (For size comparison my daughter who was the same age as the cabbage is shown.The small plant in my daughter’s hands is a summer annual variety of B. oleracea that flowers rapidly without vernalization.) Cabbage is a biennial and requires exposure to the environmental cue of prolonged winter cold in order to flower the second spring after planting. This promotion of flowering by cold is called vernalization. The large cabbage has never been vernalized and cannot flower. We are studying the biochemistry of how cold exposure promotes flowering.
Brachypodium distachyon: not vernalized left and vernalized
right; the vernalized plant is setting seed and has long ceased
vegetative growth. The plant not vernalized continues vegetive growth.