Electron Micrograph Library
Virus & Bacteriophage
Institute for Molecular Virology. University of Wisconsin -
If there are any problems please contact Ross Inman: email@example.com
Copyright © 1994-2005: These
images can be freely used for educational or research purposes, but
are not in the public domain and are not to be used for any type of
0022a.jpg: Bacteriophage lambda. At
higher magnification ( 0022b.jpg) the distal
tail spikes can clearly be observed. Method: neg. stain. Maria
0021a.jpg: Bacteriophage P2. At higher
magnification: 0021b.jpg. Method:
neg stain. Maria Schnos.
0023a.jpg: Bacteriophage P4. Method:
Neg. stain. Maria Schnos & Inman.
0023b.jpg: Bacteriophage P4 and P2. P4
phage can only infect in the presence of phage P2 and subverts P2
tail genes for its own use. Consequently the tails of both phage are
the same, but the head of phage P4 is considerably smaller because
its genome does not need to include a number of genes such as tail
genes, only the genetic code needed to steal these genes from P2. 0023c.jpg shows another comparison between both
phage. Method: Negative staining. 0023b: Maria Schnos. 0023c:
0024a.jpg: Cello Virus. Method: Neg.
phageMu.jpg: Bacteriophage Mu.
Inman, Maria Schnos and Martha Howe. Virology 1976, 72, 393.
phiX.jpg: Bacteriophage PhiX174.
Method: Neg. stain. Maria Schnos and Inman.
Wasp Virus. The Australian wasp Microplitus demolitor makes a
virus that is required for successful parasitization and egg survival.
This virus is produced in a variety of head sizes.
0024b.jpg:. DNA isolated from solutions containing such virus is
composed of double stranded DNA circles also of various sizes. Method:
negative staining. Maria Schnos and Inman.
Generalized transducing phages isolated from Listeria
monocytogenes. .The Gram-positive bacterium Listeria monocytogenes
can be found in raw food and causes human disease, The
immune-compromised are particularly susceptible, and infection
leading to listeric meningitis can be deadly. Listeria
transducing bacteriophage CU153, shown on the left has a very long tail
with two disk-like structures at the distal end (DNA content is about
42Kbp). Phage P35 shown on the right has a much shorter tail with a
single disk-like structure at the distal end. Listeria phages, More. Maria Schnos, R.Calendar and
0010a.jpg and 0010b.jpg:
Reovirus "spider". Reovirus was prepared under conditions of
gentle crosslinking, viral disruption and partial denaturation of the ds
RNA. It turns out that at least 7 of the 10 reovirus RNA segments
have unique ends attached to the residual reovirus proteins. A simpler
example of this technique, applied to phage P2, is shown in 0020a.jpg. Method: cyto-C. Maria Schnos,
Nibert & Inman.
If there are any problems contact Ross
Electron Micrograph Library
Original version: 3 May 1994. Most recent update: 30 April 2005.