Electron Micrograph Library

Virus & Bacteriophage

Institute for Molecular Virology. University of Wisconsin - Madison.

  • 0022a.jpg: Bacteriophage lambda. At higher magnification ( 0022b.jpg) the distal tail spikes can clearly be observed. Method: neg. stain. Maria Schnos.

  • 0021a.jpg: Bacteriophage P2. At higher magnification: 0021b.jpg. Method: neg stain. Maria Schnos.

  • 0023a.jpg: Bacteriophage P4. Method: Neg. stain. Maria Schnos & Inman.

  • 0023b.jpg: Bacteriophage P4 and P2. P4 phage can only infect in the presence of phage P2 and subverts P2 tail genes for its own use. Consequently the tails of both phage are the same, but the head of phage P4 is considerably smaller because its genome does not need to include a number of genes such as tail genes, only the genetic code needed to steal these genes from P2. 0023c.jpg shows another comparison between both phage. Method: Negative staining. 0023b: Maria Schnos. 0023c: Robley Williams.

  • 0024a.jpg: Cello Virus. Method: Neg. stain.

  • phageMu.jpg: Bacteriophage Mu. Inman, Maria Schnos and Martha Howe. Virology 1976, 72, 393. .

  • phiX.jpg: Bacteriophage PhiX174. Method: Neg. stain. Maria Schnos and Inman.

  • Wasp Virus. The Australian wasp Microplitus demolitor makes a virus that is required for successful parasitization and egg survival. This virus is produced in a variety of head sizes. 0024b.jpg:. DNA isolated from solutions containing such virus is composed of double stranded DNA circles also of various sizes. Method: negative staining. Maria Schnos and Inman.

  • Generalized transducing phages isolated from Listeria monocytogenes. .The Gram-positive bacterium Listeria monocytogenes can be found in raw food and causes human disease, The immune-compromised are particularly susceptible, and infection leading to listeric meningitis can be deadly. Listeria transducing bacteriophage CU153, shown on the left has a very long tail with two disk-like structures at the distal end (DNA content is about 42Kbp). Phage P35 shown on the right has a much shorter tail with a single disk-like structure at the distal end. Listeria phages,   More.   Maria Schnos, R.Calendar and R.Inman

  • 0010a.jpg and 0010b.jpg: Reovirus "spider". Reovirus was prepared under conditions of gentle crosslinking, viral disruption and partial denaturation of the ds RNA. It turns out that at least 7 of the 10 reovirus RNA segments have unique ends attached to the residual reovirus proteins. A simpler example of this technique, applied to phage P2, is shown in 0020a.jpg. Method: cyto-C. Maria Schnos, Nibert & Inman.

    If there are any problems contact Ross Inman: rbinman@wisc.edu

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    Original version: 3 May 1994.       Most recent update: 30 April 2005.