Markley Lab

People
Links
Publications

Lab Address:
Room 161,
433 Babcock Drive
Madison, WI 53706-1544
USA
(608) 262-1754

Director Address:
John Markley
Room 171A,
433 Babcock Drive
Madison, WI 53706-1544
USA
(608) 263-9349


   
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People

Graduate Students
Staff
Past Members






Graduate Students





abahrami@wisc.edu

(608) 263-9498





Anna Fuzery

fuzery@nmrfam.wisc.edu

(608) 262-4687 ext. 3117







Jin Hae Kim

jhkim23@wisc.edu

(608) 262-4687 ext. 3116



Ian Lewis

ialewis@wisc.edu

(608) 262-4687 ext. 3102





Sannali Matheson

sannalim@yahoo.com

(608) 262-4687 ext. 3104





Kuang-Lung Hsueh

hsueh@wisc.edu

(608) 262-4687 ext. 3119





Liya Wang

liyawang@wisc.edu

(608) 263-2968



Greg Zornetzer

gaz@nmrfam.wisc.edu

(608) 262-4687 ext. 3101





Staff











James Radek

jradek@nmrfam.wisc.edu

(608) 262-1754 ext. 3105





Kirsten Sorum

kasorum@wisc.edu

(608) 262-1754 ext. 3105

Past Members:

Posdoctoral fellows
Ph.D. Students
M.S. Students

Postdoctoral fellows


Masanobu Chinami
Research period:
1986-1988
Ph.D. degree:
Kurume University School of Medicine; 1982
Title of research project:
NMR titration studies of staphylococcal nuclease


Mike Reily

Research period:
1987-1988
Ph.D. degree:
Emory University; 1987
Title of research project:
NMR studies of cytochrome c553


Mark E. Anderson

Research period:
1987-1990
Ph.D. degree:
Montana State University, Bozeman, MT; 1987
Title of research project:
Physiological NMR spectroscopy


Michael Zehfus

Research period:
1987-1990
Ph.D. degree:
Pennsylvania State Hershey Medical Center; 1987
Title of research project:
NMR studies of cytrochrome c553


Prashanth Darba

Research period:
1989-1991


Charles McNemar

Research period:
1989-1991
Ph.D. degree:
Pennsylvania State University, College Park, PA; 1989
Title of research project:
NMR studies of cytochrome c553


Lars Skjeldal

Research period:
1989-1991
Ph.D. degree:
University of Trondheim, Norway; 1989
Title of research project:
NMR studies of ferredoxins


Roger A. Chylla

Research period:
1990-1993
Ph.D. degree:
University of Illinois, Urbana-Champaign; 1990
Title of research project:
Algorithms for processing protein NMR data


Zeljko, Dzakula

Research period:
1990-1993
Ph.D. degree:
University of Belgrade, Yugoslavia; 1991
Title of research project:
NMR analysis of mobile regions of protein structures


Frits Abildgaard

Research period:
1991-1993
Ph.D. degree:
University of Copenhagen, Denmark; 1991
Title of research project:
Multinuclear, multidimensional NMR spectroscopy of proteins


Hong Cheng

Research period:
1991-1994
Ph.D. degree:
Hunter College, City College of New York; 1991
Title of research project:
NMR studies of iron-sulfur proteins


Honggao Yan

Research period:
1991-1994
Ph.D. degree:
Ohio State University, Columbus, OH; 1991
Title of research project:
NMR spectroscopy of proteins


Zsolt Zolnai

Research period:
1993-1995
Ph.D. degree:
University of Belgrade, Yugoslavia; 1992
Title of research project:
NMR spectroscopy


Barbara Lewis

Research period:
1993-1996


Jurgen Schleucher

Research period:
1994-1997
Ph.D. degree:
University of Frankfurt, Germany; 1994
Title of research project:
NMR pulse sequence development


Brian F. Volkman

Research period:
1995-2000
Ph.D. degree:
University of California at Berkeley; 1994
Title of research project:
NMR spectroscopy of proteins


Henrik W. Anthonsen

Research period:
1996-1997
Ph.D. degree:
Trondheim University, Norway; 1995
Title of research project:
NMR spectroscopy of proteins


Fariba Assadi-Porter

Research period:
1998-1999, 2000
Ph.D. degree:
Biomolecular Chemistry Department, University of Wisconsin-Madison
Title of research project:
Sweet protein and sweet taste receptor


Hikaru Hemmi

Research period:
1998-1999
Ph.D. degree:
Tohoku University, Japan
Title of research project:
Solution structure of an effector protein in toluene-4-monooxygenase complex (T4MOD)


Wen-Jin Wu

Research period:
1999- 2003
Ph.D. degree:
State University of New York, Stony Brook; 1999


Timothy Machonkin

Research period:
2000-2003
Ph.D. degree:
Stanford University; 2000
Title of research project:
NMR investigations of iron-sulfur proteins and other metal-containing proteins


Claudia C. Cornilescu

Research period:
2002-2004
Ph.D. degree:
University of Maryland; 2002


John L. Newman

Research period:
2003-2005
Ph.D. degree:
University of Maryland; 2003
Title of research project:
NMR studies on the oxidized form of T4moC; Studies on the VKORC1 membrane protein


Ph.D. students


Michael A. Prubcan
Training period: 1973-1978
Previous degree: B.S. in Chemistry, St. Norbert College, 1971
Title of research project: Nuclear Magnetic Resonance Studies of Serine Proteinases and Proteinase Inhibitors

Ph.D. thesis, Purdue University, 1978


Ignacio B. Ilbanez
Training period: 1974-1980
Previous degree: Chemist diploma, University of Chile, 1973
Title of research project: Nuclear Magnetic Resonance Studies of the Active Site of Bovine Chymotrypsinogen A and Chymotrypsin A2

Ph.D. thesis, Purdue University, 1980


William M. Westler
Training period: 1975-1980
Previous degree: B.S. in Chemistry, Grove City College, 1972; M.S. in Chemistry, John Carroll University, 1974
Title of research project: Nuclear Magnetic Resonance Studies of a-Lytic Proteinase, a Bacterial Serine Proteinase

Ph.D. thesis, Purdue University, 1980


David H. Croll
Training period: 1976-1982
Previous degree: B.A. in chemistry, University of Montana, 1971
Title of research project: Nuclear Magnetic Resonance Studies of Avian Ovomucoids and Their Isolated Domains

Ph.D. thesis, Purdue University, 1980


Tse-Ming Chan
Previous degree: B.S. in chemistry and physics, University of Hong Kong; M.S. in chemistry, University of Missouri-Kansas City
Title of research project: Nuclear Magnetic Resonance Studies of Plant-Type Ferredoxins


Gyung-Ihm Rhyu
Training period: 1980-1984
Previous degree: B.S. in chemistry, Sogang University, Seoul, Korea
Title of research project: Multinuclear Magnetic Resonance Studies of Phosphoglucomutase

Ph.D. thesis, Purdue University, 1984


Christopher L. Kojiro
Training period: 1977-1985
Previous degree: B.S. in chemistry, Purdue University, 1976
Title of research project: Nuclear Magnetic Resonance Studies of the Blue Copper Protein Plastocyanin

Ph.D. thesis, Purdue University, 1985


Brian Tobias
Training period: 1981-1986
Previous degree: B.A. in chemistry, University of Arizona
Title of research project: Nuclear Magnetic Resonance Studies on Serine Proteinases

Ph.D. thesis, Purdue University, 1986


Andrew D. Robertson
Training period: 1985-1988
Previous degree: B.S. in biochemistry, University of California, San Diego
Title of research project: Structure and Conformational Dynamics of Turkey Ovomucoid Third Domain by Nuclear Magnetic Resonance Spectroscopy

Ph.D. degree, University of Wisconsin-Madison, 1988


Andrei T. Alexandrescu
Training period: 1984-1989
Previous degree: B.A. in chemistry, City College of New York, 1983
Title of research project: Nuclear Magnetic Resonance Spectroscopy of Staphylococcal Nuclease: Effects of Solution Conditions and Sequence Changes on Conformational Forms

Ph.D. degree, University of Wisconsin-Madison, 1989


Brian J. Stockman
Training peridod: 1985-1989
Previous degree: B.S. in chemistry, University of Iowa, 1985
Title of research project: Multinuclear NMR Studies of the Flavodoxin from Anabaena 7120: b-Sheet Structure and the Flavin Mononucleotide Binding Site


Byung-Ha Oh
Training period: 1985-1989
Previous degree: B.Sc. in agriculture, Seoul National University, 1983; M.S. in food science (Enzyme Technology Laboratory), Seoul National University, 1985
Title of research project: Multinuclear NMR Studies of the Plant-Type Ferredoxin from Vegetative Anabaena sp. strain PCC 7120

Ph.D. thesis, University of Wisconsin-Madison


Andrew P. Hinck
Training period: 1987-1993
Previous degree: B.S. in chemistry, University of Puget Sound, 1987
Title of research project: NMR Investgations of a Model Proline Cis:Trans Isomerization Reaction in Staphylococcal Nuclease


Stewart N. Loh
Training period: 1987-1995
Previous degree: B.S. in chemistry, University of Utah, 1987
Title of research project: Conformational Equilibria in Proteins: Hydrogen Exchange and Isotope Fractionation Studies of Staphylococcal Nuclease


William F. Walkenhorst
Training period: 1986-1993
Previous degree: B.S. in chemistry, Bradley University, 1983
Title of research project: Structural Investigations of Ovomucoid Third Domains by Nuclear Magnetic Resonance Methods


Andrzej M. Krezel
Training period: 1987-1991
Previous degree: M.S. in biology (with specialization in molecular biology), University of Warsaw, 1986
Title of research project: Solution Structure Determination of Turkey Ovomucoid Third Domain by Nuclear Magnetic Resonance Techniques

Ph.D. thesis, University of Wisconsin-Madison


Arthur S. Edison
Training period: 1989-1993
Previous degree: B.S. in chemistry, University of Utah, Salt Lake City, Utah
Title of research project: Theoretical and Experimental Investigations of Peptides and Proteins by NMR Spectroscopy


Young Kee Chae
Training period: 1988-1994
Previous degree: B.S. in physics
Title of research project: Multinuclear NMR Studies of the Heterocyst Ferredoxin from Anabaena 7120


Hua Zha
Training period: 1992-1994
Previoud degree: M.S. in physics, Montana State University
Title of research project: Pulse Techniques in NMR Spectroscopy


Charles G. Hoogstraten
Training period: 1990-1995
Previous degree: B.S. in chemistry, Michigan State University
Title of research project: Removal of Errors due to Spin diffusion from NMR Distance Measurements and Protein Solution Structures: an Experimental Approach


John B. Olson, Jr.
Training period: 1989-1995
Previous degree: B.S., College of Charleston, SC
Title of research project: A General and Adaptable Method for the Automated Assignment of Protein Multidimensional Nuclear Magnetic Resonance Spectra


Ben Xia
Training period: 1991-1996
Previous degree: B.S. in physiology and Biophysics, Peking University, China
Title of research project: Multinuclear NMR and Mutagenesis Studies of Human Ferredoxin


Soheui Choe
Training period: 1991-1997
Previous degree: B.S. in chemistry, Seoul National University, Seoul, Korea
Title of research project: Overexpression and Isotope Labeling of Ovomucoid Third Domains and their Investigation by NMR Spectroscopy


Kenneth E. Prehoda
Training period: 1991-1997
Previous degree: B.A. in chemistry, California State University-Sacramento, California
Title of research projet: Pressure Effects on Protein Structure and Dynamics


Jane Caldwell
Training period: 1992-1997
Previous degree: B.S., Northwestern University
Title of research project: Solution Structure of Brazzein


Dagmar Truckses
Training period: 1991-1998
Previous degree: B.A. in biophysics, University of California, Berkeley, California
Title of research project: Investigation of Mutants of Staphylococcal Nuclease with Altered Stability and Dynamic Properties


Michelle DeRider
Training period: 1995-2001
Previous degree: B.A., Macalester College
Title of research project: Conformational Analysis of Peptides and Proteins


Steven Wilkens
Training period: 1996-2001
Previous degree:
Title of research project: High-Level Quantum Mechanical Computations of Chemical Shifts in Iron-Sulfur Proteins




Qin (Kate) Zhao
Training period: 1996-2001
Title of research project: Solution NMR Studies of ATT, an Arabidopsis thaliana Trypsin/Chymotrypsin Inhibitor




E. Sonay Elgin
Training period:
Title of research project: Structure-Function Studies of the Human Chemokine Lymphotactin and its Equilibrium Between Two Highly Divergent Conformational States/em>


Jikui Song
Training period: 1997-2002
Title of research project: Structure-Function Investigations of Proteinase Inhibitors and Proteinases


Heike Blad
Training period:
Title of research project: Dynamics of Biological Marcormolecules as Probed by NMR


I-Jin Lin
Training period: 2000-2005
Title of research project: NMR spectroscopic studies of iron-sulfur proteins: rubredoxin from Clostridium pasteurianum and Rieske protein from Thermus thermophilis


M.S. students


Sum May Cheung
Training period: 1972-1974
Previous degree: B.Sc., Chemistry, University of California, Santa Barbara, 1972
Title of research project: On the Assignment of the Histidine NMR Peaks of Ribonuclease A by Hydrogen Exchange Techniques

M.S. Thesis, Purdue Univerity, 1974


Xinjie Wu
Training period: 1981-1983
Previous degree: Diploma in chemistry (#(67)-578), Nanjing University, 1967
Title of research project: One- and Two-Dimensional 470 MHz QH NMR Studies of Amino Acids, Water, Some Ammonium Salts, and Turkey Ovomucoid Third Domain

M.S. Thesis, Purdue University, 1983


Gilberto Ortiz-Polo
Training period: 1981-1985
Previous degree: B.S. in chemistry, University of Puerto Rico, Rio Piedras
Title of research project: Nuclear Magnetic Resonance Studies of Turkey Ovomucoid Third Domain Variants

M.S. Thesis, Purdue University, 1985


Bin Yuan
Training period: 1986-1988
Previous degrees: B.S. in chemistry, Zhejiang University, China

M.S. in biochemistry, Shanghai Biochemistry Institute, 1986
Title of research project: NMR study of 15N Enriched Staphylococcal Nuclease

M.S. Thesis, University of Wisconsin-Madison


Ross J. Reedstrom
Training period: 1987-1992
Previous degree: B.A., Yale University
Title of research project: Protein overproduction and isolation for NMR and X-ray studies


Marian Toscano
Training period: 1994-1995
Previous degree: B.S. in biochemistry, University of Wisconsin-Madison
Title of research project: Inherent Instability of a Synthetic Tetrameric Peptide

University of Wisconsin-Madison, 1995


Biao Sun
Training period: 1993-1995
Previous degree: M.S. in physics, University of Pittsburgh
Title of research project: NMR Spectroscopy of [2Fe-2S] Ferredoxins


Mike Birkeland
Training period:
Title of research project:

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