Papers and Supplementary Material

Goldstrohm AC, Wickens M. Multifunctional deadenylase complexes diversify mRNA control.
Nat Rev Mol Cell Biol. 2008 Apr;9(4):337-44.  [Pub Med]

Wickens M, Kwak JE. Molecular biology. A tail tale for U. Science.
2008 Mar7;319(5868):1344-5  [Pub Med]

Lee MH, Hook B, Pan G, Kershner AM, Merritt C, Seydoux G, Thomson JA, Wickens M, Kimble J.
Conserved regulation of MAP kinase expression by PUF RNA-binding proteins.
PLoS Genet. 2007 Dec 28;3 (12).  [Pub Med]

Coller J, Wickens M. Tethered function assays: and adaptable approach
to study RNA regulatory proteins. Methods enzymol. 2007;429:299-321.  [Pub Med]

Kwak JE, Wickens M.  A family of poly(U) polymerases. RNA. 2007 Jun;
13(6):680-7. epub 2007 apr 20.  [Pub Med]

Hook BA, Goldstrohm AC, Seay DJ, Wickens M. Two yeast PUF proteins
negatively regulate a single mRNA. J Biol Chem. 2007 May 25;282(21):15430-8.
Epub 2007 Mar 27.  [Pub Med]

Rouhana L, Wickens M.  Autoregulation of GLD-2 cytoplasmic poly (A)
polymerase. RNA. 2007 Feb;13(2):188-99. Epub 2006 Dec12.  [Pub Med]

Goldstrohm AC, Seay DJ, Hook BA, Wickens M.  PUF protein-mediated
is catalyzed by Ccr4p.  J Biol Chem. 2007 Jan 5;282(1):109-14. Epub 2006 Nov 7.  [Pub Med]

Daniel Seay, Brad Hook, Katie Evans and Marvin Wickens.  A three-hybrid screen identifies mRNAs controlled by a regulatory protein. RNA. Aug;12(8):1594-600. Epub 2006 Jun 29. [Pub Med]

Lee, M.-H., B. Hook, L.B. Lamont, M. Wickens and J. Kimble.  LIP-1 phosphatase controls the extent of germline proliferation in Caenorhabditis elegans. EMBO J, 25, 88-96. [Pub Med]

Goldstrohm, A.C., B.A. Hook, D.J. Seay and M. Wickens.  PUF proteins bind Pop2p to regulate messenger RNAs. Nature Structural & Molecular Biology. Published online. [Pub Med]

Opperman, L., B. Hook, M. DeFino, D.S. Bernstein, and M. Wickens.  A single spacer nucleotide determines the specificities of two mRNA regulatory proteins. Nature Structural & Molecular Biology. 12, 945-951. [Pub Med]

Rouhana, L, L. Wang, N. Buter, J.E. Kwak, C.A. Schiltz, T. Gonzalez, A.E. Kelley, C.F. Landry, and M. Wickens.  Vertebrate GLD2 poly(A) polymerases in the germline and the brain. RNA, 11, 1117-1130. [Pub Med]

Thompson, B.E., D. Bernstein, J. Bachorik, A. Petcherski, M. Wickens, and J. Kimble.  Dose-dependent control of proliferation and sperm specification by FOG-1/CPEB. Development, 132, 3471-3481. [Pub Med]

David Bernstein, Brad Hook, Ashwin Hajarnavis, Laura Opperman, and Marvin Wickens.  Binding specificity and mRNA targets of a C. elegans PUF protein, FBF-1. RNA, 11:447–458. [Pub Med]

Hook, B., D. Bernstein, B. Zhang, and M. Wickens.  RNA-protein interactions in the yeast three-hybrid system: affinity, sensitivity and enhanced library screening. RNA, 11, 227-233.[Pub Med]

Lamont, L.B., S.L. Crittenden, D. Bernstein, M. Wickens, J. Kimble.  FBF-1 and FBF-2 regulate the size of the mitotic region in the C. elegans germline. Dev. Cell. 7, 697-707.[Pub Med]

M. Wickens and Tania N.Gonzalez. Knives,Accomplices,and RNA. Science 30619. 2004 Nov: 1299-1300 [Pub Med]

Wang L, Kimble J, Wickens M. Tissue-specific modification of gld-2 mRNA in C. elegans: likely C-to-U editing. RNA. 2004 Sep;10(9):1444-8. [Pub Med]

Kwak JE, Wang L, Ballantyne S, Kimble J, Wickens M. Mammalian GLD-2 homologs are poly(A) polymerases. Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4407-12. [Pub Med]

Eckmann CR, Kraemer B, Wickens M, Kimble J. GLD-3, a bicaudal-C homolog that inhibits FBF to control germline sex determination in C. elegans. Dev Cell. 2002 Nov;3(5):697-710. [Pub Med]

S. Butcher and M. Wickens. 2004. Star-studded circuitry. Nature Structural and Molecular Biology 11, 2-3. [Pub Med]

M. Wickens and A. Goldstrohm. 2003. A place to die, a place to sleep. Science 300, 753-755. [Publisher's Link][Pub Med]

CR Eckmann, B. Kraemer, M. Wickens, J. Kimble. 2002. GLD-3, a bicaudal-C homolog that inhibits FBF to control germline sex determination in C. elegans. Dev Cell. 2002 Nov;3 (5):697-710. [Pub Med]

M. Wickens, D. Bernstein, S. Crittenden, C. Luitjens and J. Kimble. 2001. PUF proteins and 3'UTR regulation in the C. elegans germline. In Cold Spring Harbor Symposium Volume, Cold Spring Harbor Laboratory Press, Woodbury, NY.

Sarah L. Crittenden, David S .Bernstein, Jennifer L. Bachorik, Beth E. Thompson, Maria Gallegos, Andrei G. Petcherski, Gary Moulder, Robert Barstead, Marvin Wickens, and Judith Kimble. 2002. A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans. Nature 417, 660-664 [Pub Med]

Liaoteng Wang, Christian R. Eckmann, Lisa C. Kadyk, Marvin Wickens and Judith Kimble . 2002. A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans. Nature 419, 312-316 [Pub Med]

Jeffery Coller and Marvin Wickens. 2002. Tethered function assays using 3(') untranslated regions. Methods 2, 142-50 [Pub Med]

David Bernstein, Natascha Buter, Craig Stumpf and Marvin Wickens. 2002. Analyzing mRNA-protein complexes using a yeast three-hybrid system: methods and applications. Methods 2, 123-41 [Pub Med]

Marvin Wickens, David S. Bernstein, Judith Kimble and Roy Parker. 2002. A PUF family portrait: 3'UTR regulation as a way of life. Trends in Genetics 18, 150-156. [Supplement]

K. Dickson, S. R. Thompson, N. K. Gray and M. Wickens. 2001 Poly(A) polmerase and the regulation of cytoplasmic polyadenylation. J Biol Chem 276, 41810-41816. [PubMed]

C. Luitjens, M. Gallegos, B. Kraemer, J. Kimble and M. Wickens. 2000. CEPB proteins control two key steps in spermatogenesis in C. elegans. Genes Dev. 14, 2596-2609. [PubMed]

Marvin Wickens, Elizabeth B. Goodwin, Judith Kimble, Sidney Strickland, and Matthias Hentze. Translational Control in Developmental Decisions. In Translational Control Second Edition. (Michael Mathews, ed.) Cold Spring Harbor Press, New York, pp. 295-370.

Brian Kraemer, Beilin Zhang, Dhruba SenGupta, Stanley Fields and Marvin Wickens, In Press. A yeast three-hybrid system to detect and analyze interactions between RNA and protein. In RNA-Ligand Interactions. (Daniel W. Celander and John N. Abelson, eds.) of the Methods in Enzymology Series. Academic Press, New York. Vol. 328: Part III, pp.

N. K. Gray, J.M. Coller, K. Dickson and M. Wickens. Multiple portions of poly(A)-binding protein stimulate translation in vivo. EMBO J. 19, pp. 4723-4733. [Supplement] [PubMed]

A. F. Barkoff, K.S. Dickson, N.K. Gray and Marvin Wickens. 2000. Translational control of cyclin B1 mRNA during meiotic maturation: coordinated repression and cytoplasmic polyadenylation. Developmental Biology 220, 97-109. [PubMed]

S. R. Thompson, E.B. Goodwin and M. Wickens. 2000. Rapid deadenylation and poly(A)-dependent translational repression mediated by the C. elegans tra-2 3’UTR in Xenopus embryos. Mol & Cell Biol. 20, 2129-2137. [PubMed]

Beilin Zhang, Brian Kraemer, Dhruba SenGupta, Stanley Fields and Marvin Wickens. 1999. A three-hybrid system to detect and analyze RNA-protein interactions in vivo. In Expression of Recombinant Genes in Eukaryotic Systems. (J. Glorioso and M. Schmidt, eds.) of the Methods in Enzymology Series. Academic Press, New York. Vol. 306, pp. 93-113.

B. Kraemer, S. Crittenden, M. Gallegos, G. Moulder. R. Barstead, J. Kimble and M. Wickens. 1999. NANOS-3 and FBF proteins physically interact to control the sperm/oocyte switch in C. elegans. Current Biology 9, 1009-1018. [PubMed]

Kirsten Dickson, S., Bilger, A., Ballantyne, Scott and Wickens, Marvin. 1999. CPSF in X. laevis oocytes: A cytoplasmic factor involved in regulated polyadenylation. Mol. & Cell. Biol. 19, 5707-5717. [PubMed]

Dhruba J. SenGupta, Marvin Wickens and Stanley Fields, 1999. Identification of RNAs that bind to a specific protein using the yeast three-hybrid system. RNA 5, 596-601. [PubMed]

Eun-gyung Lee, Ashley Yeo, Brian Kraemer, Marvin Wickens and Maxine L. Linial. 1999. The Gag domains required for avian retroviral RNA encapsidation determined by using two independent assays. Journal of Virology 73, 6282-6292. [PubMed]

Nicola Gray and Marvin Wickens. 1998. Control of translation initiation in animals. In Ann. Rev. Cell Dev. Biol. (James A. Spudich, editor) Annual Reviews, Inc., Palo Alto, CA Vol. 14, pp. 399-458. [PubMed]

Beilin Zhang, Brian Kraemer, Dhruba SenGupta, Stanley Fields and Marvin Wickens. 1998. A three-hybrid system to detect and analyze RNA: protein interactions in vivo. In RNA-Protein Interactions: A Practical Approach. (Chris Smith, editor) Oxford University Press, Oxford, England, pp. 195-216.

Donald L. Gillian-Daniel, Nicola K. Gray, Jonas Astrom, Aaron Barkoff and Marvin Wickens. 1998. Modifications of the 5' cap of mRNAs during Xenopus oocyte maturation: independence from changes in poly(A) length and impact on translation. Mol. & Cell. Biol. 18, 6152-6163. [PubMed]

Jeffrey M. Coller, Nicola K. Gray and Marvin Wickens. 1998. mRNA stabilization by poly(A) binding protein is independent of poly(A) and requires translation. Genes Dev. 12, 3226-3235. [PubMed]

Barkoff, A, Ballantyne, S. and Wickens, M. 1998. Polyadenylation of multiple mRNAs is required for oocyte maturation. EMBO J. 17, 3168-3175. [PubMed]

Richard J. Jackson and Marvin Wickens. 1997. Translational controls impinging on the 5'-untranslated region and initiation factor proteins. In Current Opinion in Genetics & Development (Ron Laskey and Matthew P. Scott eds.) Current Biology Ltd., London, U.K. Vol. 7, pp. 233-241. [PubMed]

Marvin Wickens, Philip Anderson and Richard J. Jackson. 1997. Life and death in the cytoplasm: messages from the 3'end. In Current Opinion in Genetics & Development (Ron Laskey and Matthew P. Scott eds.) Current Biology Ltd., London, U.K., Vol. 7, pp. 220-232. [PubMed]

Beilin Zhang, Brian Kraemer, Dhruba SenGupta, Stanley Fields and Marvin Wickens. 1997. A three-hybrid system to detect and analyze RNA-protein interactions in vivo. In The Yeast Two-Hybrid System (P.L. Bartel and Stanley Fields, eds.) Oxford University Press, New York, New York, pp. 298-315.

A. Puoti, M. Gallegos, B. Zhang, M.P. Wickens and J. Kimble. 1997. Controls of cell fate and pattern by 3' untranslated regions: The Caenorhabditis elegans sperm/oocyte decision. Cold Spring Harbor Symposia on Quantitative Biology, 62, 19-24. [PubMed]

Beilin Zhang, Maria Gallegos, Alessandro Puoti, Eileen Durkin, Stanley Fields, Judith Kimble, and Marvin Wickens. 1997. A conserved RNA binding protein that regulates patterning of sexual fates in the C. elegans hermaphrodite germ line. Nature 390, 477-484. [PubMed]

Scott Ballantyne, Donald Daniel, Jr., and Marvin Wickens. 1997. A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation. Mol. Biol. Cell 8, 1633-1648. [PubMed]

Susan McCracken, Nova Fong, Krassimir Yankulov, Scott Ballantyne, Guohua Pan, Jack Greenblatt, Scott D. Patterson, Marvin Wickens and David L. Bentley. 1997. The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. Nature 385, 357-361. [PubMed]

Marvin Wickens, Joel G. Belasco, and Allan Jacobson. 1996. Changes in the length of poly(A) tails and their effects on mRNA translation and turnover. In Nato ASI Series (Orna Resnekov and Alexander von Gabain, eds.) Springer-Verlag, Berlin, Heidelberg, pp. 45-55.

Marvin Wickens, Judith Kimble and Sidney Strickland. 1996. Translation regulation of developmental decisions. In Translational Control (Michael Mathews, ed.) Cold Spring Harbor Press, New York, pp. 411-450.

Arturo Verrotti, Sunnie Thompson, Christopher Wreden, Sidney Strickland and Marvin Wickens. 1996. Evolutionary conservation of sequence elements controlling cytoplasmic polyadenylylation. Proc. Natl. Acad. Sci. 93, 9027-9032. [PubMed]

Dhruba SenGupta, Beilin Zhang, Brian Kraemer, Pascale Prochart, Stanley Fields and Marvin Wickens. 1996. A three-hybrid system for detecting RNA-protein interactions. Proc. Natl. Acad. Sci. 93, 8496-8501. [PubMed]

Michael D. Sheets, Michael Wu and Marvin Wickens. 1995. Polyadenylation as a control point in Xenopus meiotic maturation. Nature 374, 511-516. [PubMed]

Scott Ballantyne, Andrea Bilger, Anders Virtanen and Marvin Wickens. 1995. Poly (A) polymerases in the nucleus and cytoplasm of frog oocytes: dynamic regulation during oocyte maturation and early development. RNA 1, 64-78. [PubMed]

Marvin Wickens and Kathy Takayama. 1994. Deviants or emissaries. Nature 367, 17-18.

Andrea Bilger, Catherine A. Fox, Elmar Wahle and Marvin Wickens. 1994. Nuclear polyadenylation factors recognize cytoplasmic polyadenylation elements. Genes Dev. 8, 1106-1116. [PubMed]

Michael D. Sheets, Catherine A. Fox, Tim Hunt, George Vande Woude and Marvin Wickens. 1994 The 3'UTRs of c-mos and cyclin mRNAs contol translation by regulating cytoplasmic polyadenylation. Genes Dev. 8, 926-938. [PubMed]

Marvin Wickens. 1993. Springtime in the desert. Nature 363, 305-306.

Marvin Wickens. 1992. RNA and the early embryo. Seminars in Developmental Biology 3, 363-366.

Marvin Wickens. 1992. Forward, backward, how much, when: Mechanisms of poly(A) addition and removal and their role in early development. Seminars in Developmental Biology. 3, 399-412.

Catherine Fox, Michael Sheets, Elmar Wahle and Marvin Wickens. 1992. Polyadenylation of maternal mRNA during oocyte maturation: poly(A) addition in vitro requires a regulated RNA binding activity and a poly(A) polymerase. EMBO J. 11, 5021-5032. [PubMed]

Wayne Forrester, Francoise Stutz, Michael Rosbash, and Marvin Wickens. 1992. Defects in mRNA 3'-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure. Genes Dev. 6, 1914-1926. [PubMed]

Vivian Bardwell, Marvin Wickens, Silke Bienroth, Walter Keller, Brian Sproat and Augus Lamond. 1991. Site-directed ribose methylation identifies 2'-OH groups in polyadenylation substrates critical for AAUAAA recognition and poly(A) addition. Cell 65, 125-133. [PubMed]

Martin Fleischmann, Michel Clark, Wayne Forrester, Marvin Wickens, Takeharu Nishimoto and Markus Aebi. 1991. Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1. Mol. Gen. Gen. 227, 417-423. [PubMed]

Marvin Wickens. 1990. In the beginning is the end: Regulation of poly(A) addition and removal during early development. Trends in Biochem. Sci. 15, 320-324.

Marvin Wickens. 1990. How the messenger got its tail: Addition of poly(A) in the nucleus. Trends in Biochem. Sci. 15, 277-281.

Stephen Ogg, Philip Anderson and Marvin Wickens, 1990. Splicing of a C. elegans pre-mRNA in a mammalian nuclear extract. Nucleic Acids Res. 18, 143-149.

Vivian Bardwell, David Zarkower, Mary Edmonds and Marvin Wickens. 1990. The enzyme that adds poly(A) to mRNAs is a classical poly(A) polymerase. Mol. Cell Biol. 10, 846-849.

Vivian Bardwell and Marvin Wickens. 1990. Polyadenylation-specific complexes undergo a transition early during the addition of a polyA tail. Mol. Cell Biol. 10, 295-302.

Peter Wigley, Michael Sheets, David Zarkower, Mary Whitmer and Marvin Wickens. 1990. Polyadenylation of mRNA: minimal substrates and a requirement for the 2' hydroxyl of the U in AAUAAA. Mol. Cell Biol. 10, 1705-1713.

Vivian Bardwell and Marvin Wickens. 1990. Purification of RNA and RNA-protein complexes by R17coat protein affinity chromatography. Nucleic Acids Res. 18, 6587-6594.

Michael Sheets, Stephen Ogg and Marvin Wickens. 1990. Point mutations in AAUAAA and poly(A) addition site: effects on the accuracy and efficiency of cleavage and polyadenylation in vitro. Nucleic Acids Res. 18, 5799-5805.

Catherine Fox and Marvin Wickens. 1990. Poly(A) removal during oocyte maturation: a default reaction selectively prevented by specific sequences in the 3' UTR of certain maternal mRNAs. Genes Dev. 4, 2287-2298.

Michael D. Sheets and Marvin Wickens. 1989. Two phases in the addition of a polyA tail. Genes Dev. 3, 1401-1412.

Catherine Fox, Michael D. Sheets and Marvin Wickens. 1989. PolyA tail addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU. Genes Dev. 3, 2151-2162.

Laura Conway and Marvin Wickens. 1989. Modification interference, in Methods in Enzymology 180, 369-379.

David Zarkower and Marvin Wickens. 1988. A functionally redundant downstream sequence is required for mRNA 3' end formation and for assembly of a pre-cleavage complex in vitro. J. Biol. Chem. 263, 5780-5788.

David Zarkower and Marvin Wickens. 1987. Formation of mRNA 3' termini: stability and dissociation of a complex involving the AAUAAA sequence. EMBO J. 6, 177-186.

Michael D. Sheets, Pauline Stephenson and Marvin Wickens. 1987. The products of
cleavage and polyadenylation of SV40 late pre-mRNAs in vitro. Mol. Cell Biol. 8, 1518-1529.

Laura Conway and Marvin Wickens. 1987. Analysis of mRNA 3' end formation by modification interference: the only modifications which prevent processing lie in AAUAAA nd the poly(A) Site. EMBO J. 6, 4177-4184.

David Zarkower and Marvin Wickens. 1987. Specific pre-Cleavage and post-cleavage complexes involved in the formation of SV40 late mRNA 3' termini in vitro. EMBO J. 6, 4185-4192.

Marvin P. Wickens and James E. Dahlberg. 1987. RNA-protein interactions. Cell 51, 339-342.

David Zarkower, Pauline Stephenson, Michael D. Sheets and Marvin P. Wickens. 1986. The AAUAAA Sequence is required both for cleavage and for polyadenylation of Simian Virus 40 pre-mRNA in vitro. Mol. Cell Biol. 6, 2317-2323.

Laura Conway and Marvin P. Wickens. 1985. A sequence downstream of AAUAAA is required for formation of simian virus 40 late mRNA 3' termini in frog oocytes. Proc. Natl. Acad. Sci. 82, 3949-3953.

Marvin Wickens and Pauline Stephenson. 1984. Role of the conserved AAUAAA sequence: four AAUAAA point mutatiuons prevent mRNA 3' end formation. Science 226, 1045-1051.

Marvin Wickens and J. B. Gurdon. 1983. Post-transcriptional processing of SV40 late transcripts in injected frog oocytes. J. Mol. Biol. 163, 1-26.

J. B. Gurdon and Marvin P. Wickens. 1983. The use of Xenopus oocytes for the expression of cloned genes in Methods in Enzymology, 101, Academic Press, pp. 370-386.

S. Weisbrod, Marvin P. Wickens, S. Whytock and J. B. Gurdon. 1982. Active chromatin of oocytes injected with somatic cell nuclei or cloned DNA. Dev. Biol. 94, 216-229.

Marvin P. Wickens and Ronald A. Laskey. 1981. Expression of cloned genes in cell-free systems and in microinjected Xenopus oocytes, in Vol. 1, Genetic Engineering, ed. Robert Williamson, Academic Press, London.

Marvin Wickens and Ronald A. Laskey. 1981. Expression of cloned genes in cell-free systems and in microinjected Xenopus oocytes, in Vol. 1, Genetic Engineering, ed. Robert Williamson, Academic Press, London.

Marvin Wickens, S. L. Woo, B. W. O'Malley and J. B. Gurdon. 1980. Expression of a chicken ovalbumin gene injected into frog oocyte nuclei. Nature 285, 628-634.

Gary N. Buell, Marvin Wickens, John Carbon and Robert T. Schimke. 1979. Isolation of recombinant plasmids bearing cDNA to hen ovomucoid and lysozyme mRNAs. J. Biol. Chem. 254, 9277-9283.

Marvin Wickens, Gary N. Buell, Gray F. Crouse, John Carbon and Robert T. Schimke. 1979. Restriction map of the region surrounding the EcoRI site in the pCR1 plasmid and analysis of an inserted ovabumin Gene 5, 19-43.

Marvin Wickens, Gary N. Buell and Robert T. Schimke. 1978. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid and ovalbumin mRNAs: optimization for full length second strand synthesis by E. coli DNA polymerase I. J. Biol. Chem. 253, 2483-2495.

Gary N. Buell, Marvin Wickens, Farhang Payvar and Robert T. Schimke. 1978. Synthesis of full length cDNAs from four partially purified oviduct mRNAs. J. Biol. Chem. 253, 2471-2482.

Anne d'Albis, Marvin Wickens and W. B. Gratzer. 1975. Base-pairing of adenine and uracil derivatives in the presence of water. Biopolymers 14, 1423-1435.